Dr. Anthony Campagnari Microbiology and Immunology Professor of Microbiology 143 Biomedical Research Building Buffalo NY, 14214 Phone: (716) 829-2673 Email: aac@buffalo.edu View map

DESCRIPTION OF INTERESTS

My research interests are primarily in microbial pathogenesis, specifically bacterial virulence factors. Currently, there are three NIH funded research projects underway in my laboratory. The first involves the lipooligosaccharides (LOS) of Haemophilus ducreyi. This sexually transmitted pathogen causes chancroid, which is now recognized as a cofactor in the heterosexual transmission of the HIV. Our efforts are directed toward defining a role for the LOS in the early stages of pathogenesis. Our studies suggest that this glycolipid is involved in adherence and invasion of human keratinocytes, which may play a critical role in colonization. A principle goal of this work is to determine which region(s) of the LOS is directly involved in attachment. A second goal is to identify LOS receptors on human cells. These studies will provide a better understanding of the steps involved in pathogenesis, which will provide the data needed for designing specific strategies aimed at preventing infection. Our second project involves the study of iron-regulated proteins as vaccine candidates against the respiratory pathogen Moraxella catarrhalis. This human mucosal pathogen is a major cause of otitis media in children and also causes exacerbations in adults with chronic obstructive pulmonary disease (COPD). We have identified 2 proteins, which appear to be conserved on all strains of M. catarrhalis. Our data suggests that these proteins are expressed in vivo and immunogenic. In addition, our data shows that these proteins are receptors for human transferrin or lactoferrin. One aim of this grant are to analyze the human immune response to each of these proteins. Both the serum response and the mucosal response will be evaluated for antibody reactivity and biologic activity. The second aim of this work is to map the two proteins on the bacterial surface. Currently we are attempting X-ray crystallographic analyses on both receptors. We hope to identify surface-exposed, conserved domains on each protein. These regions will be used to determine if these domains may be effective components of a potential vaccine to prevent M. catarrhalis infections. Our third research project involves the study of M. catarrhalis LOS. There are very few reports involving this important surface glycolipid. However, a recent study has shown that both live and heat-killed M. catarrhalis can cause otitis media-like symptoms in the middle ear of chinchillas. While this bacterium is strictly a human pathogen, this finding suggests that the LOS may be important for infection. In addition, our recent study has demonstrated that the LOS is involved in resistance to the bactericidal activity of normal human sera. Based on my expertise and that of my laboratory, we have chosen to study the role of LOS in infections caused by M. catarrhalis. Our initial efforts have focused on developing specific monoclonal antibodies that define the LOS structure(s) expressed by different serotypes of M. catarrhalis. We are also using random transposon mutagenesis to develop LOS mutants which will be analyzed in various biologic systems including the ability to adhere to human epthelium, serum sensitivity or resistance and the ability to form biofilms. These studies will provide new insight into the role of LOS in colonization and pathogenesis of middle ear disease caused by M. catarrhalis.

EDUCATION

1984	Doctor of Philosophy, Tumor Immunology SUNY@Buffalo
1981	Master of Science, Biochemistry SUNY@Buffalo (RPCI section)

EXPERTISE

Antibody development Development of monoclonal and polyclonal anitbodies to biothreat organisms.

Microbial pathogenesis Gram-negative microbial pathogenesis. Identification of colonization and virulence factors. Identification and characterization of vaccine antigens.

PUBLICATIONS

Schwingel JM, Edwards KJ, Cox AD, Masoud H, Richards JC, St Michael F, Tekwe CD, Sethi S, Murphy TF, Campagnari AA; Use of Moraxella catarrhalis lipooligosaccharide mutants to identify specific oligosaccharide epitopes recognized by human serum antibodies.; Infect Immun; 2009 Oct; 77(10); 4548-4558

Adams MD, Goglin K, Molyneaux N, Hujer KM, Lavender H, Jamison JJ, MacDonald IJ, Martin KM, Russo T, Campagnari AA, Hujer AM, Bonomo RA, Gill SR; Comparative genome sequence analysis of multidrug-resistant Acinetobacter baumannii.; J Bacteriol; 2008; 190(24); 8053-8064

Gill SR; Rat Pneumonia and Soft-Tissue Infection Models for the Study of Acinetobacter baumannii biology; Infect. Immun.; 2008; 76; 3577-3586

Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA; Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.; Infect Immun; 2008; 76(8); 3577-3586

Luke NR, Allen S, Gibson BW, Campagnari AA; Identification of a 3-deoxy-D-manno-octulosonic acid biosynthetic operon in Moraxella catarrhalis and analysis of a KdsA-deficient isogenic mutant.; Infect Immun; 2003 Nov; 71(11); 6426-6434

Furano K, Campagnari AA; Inactivation of the Moraxella catarrhalis 7169 ferric uptake regulator increases susceptibility to the bactericidal activity of normal human sera.; Infect Immun; 2003 Apr; 71(4); 1843-1848

Schilling B, Gibson BW, Filiatrault M, Campagnari AA; Characterization of lipooligosaccharides from Haemophilus ducreyi containing polylactosamine repeats.; J Am Soc Mass Spectrom; 2002 Jun; 13(6); 724-734

Tullius MV, Phillips NJ, Scheffler NK, Samuels NM, Munson Jr RS, Hansen EJ, Stevens-Riley M, Campagnari AA, Gibson BW; The lbgAB gene cluster of Haemophilus ducreyi encodes a beta-1,4-galactosyltransferase and an alpha-1,6-DD-heptosyltransferase involved in lipooligosaccharide biosynthesis.; Infect Immun; 2002 Jun; 70(6); 2853-2861

Luke NR, Karalus RJ, Campagnari AA; Inactivation of the Moraxella catarrhalis Superoxide Dismutase SodA Induces Constitutive Expression of Iron-Repressible Outer Membrane Proteins.; Infect Immun; 2002 Apr; 70(4); 1889-1895

Filiatrault MJ, Munson RS, Anthony Campagnari; Genetic Analysis of a Pyocin-Resistant Lipooligosaccharide (LOS) Mutant of Haemophilus ducreyi: Restoration of Full-Length LOS Restores Pyocin Sensitivity.; J Bacteriol; 2001 Oct; 183(19); 5756-5761

Young RS, Filiatrault MJ, Fortney KR, Hood AF, Katz BP, Munson RS, Anthony Campagnari, Spinola SM; Haemophilus ducreyi lipooligosaccharide mutant defective in expression of beta-1,4-glucosyltransferase is virulent in humans.; Infect Immun; 2001 Jun; 69(6); 4180-4184

Zaleski A, Scheffler NK, Densen P, Lee FK, Anthony Campagnari, Gibson BW, Apicella MA; Lipooligosaccharide P(k) (Galalpha1-4Galbeta1-4Glc) epitope of moraxella catarrhalis is a factor in resistance to bactericidal activity mediated by normal human serum.; Infect Immun; 2000 Sep; 68(9); 5261-5268

Filiatrault MJ, Gibson BW, Schilling B, Sun S, Munson RS, Campagnari AA; Construction and characterization of Haemophilus ducreyi lipooligosaccharide (LOS) mutants defective in expression of heptosyltransferase III and beta1,4-glucosyltransferase: identification of LOS glycoforms containing lactosamine repeats.; Infect Immun; 2000 Jun; 68(6); 3352-3361

Karalus R, Anthony Campagnari; Moraxella catarrhalis: a review of an important human mucosal pathogen.; Microbes Infect; 2000 Apr; 2(5); 547-559

Yu H, Raymonda JW, McMahon TM, Anthony Campagnari; Detection of biological threat agents by immunomagnetic microsphere-based solid phase fluorogenic- and electro-chemiluminescence.; Biosens Bioelectron; 2000 Jan; 14(10-11); 829-840

Young RS, Fortney K, Haley JC, Hood AF, Anthony Campagnari, Wang J, Bozue JA, Munson RS, Spinola SM; Expression of sialylated or paragloboside-like lipooligosaccharides are not required for pustule formation by Haemophilus ducreyi in human volunteers.; Infect Immun; 1999 Dec; 67(12); 6335-6340

Luke NR, Anthony Campagnari; Construction and characterization of Moraxella catarrhalis mutants defective in expression of transferrin receptors.; Infect Immun; 1999 Nov; 67(11); 5815-5819

Luke NR, Thomas Russo, Luther N, Anthony Campagnari; Use of an isogenic mutant constructed in Moraxella catarrhalis To identify a protective epitope of outer membrane protein B1 defined by monoclonal antibody 11C6.; Infect Immun; 1999 Feb; 67(2); 681-687

Inzana TJ, Hensley J, McQuiston J, Alan Lesse, Anthony Campagnari, Boyle SM, Apicella MA; Phase variation and conservation of lipooligosaccharide epitopes in Haemophilus somnus.; Infect Immun; 1997 Nov; 65(11); 4675-4681

Gibson BW, Anthony Campagnari, Melaugh W, Phillips NJ, Apicella MA, Grass S, Wang J, Palmer KL, Munson RS; Characterization of a transposon Tn916-generated mutant of Haemophilus ducreyi 35000 defective in lipooligosaccharide biosynthesis.; J Bacteriol; 1997 Aug; 179(16); 5062-5071

Hiltke TJ, Anthony Campagnari, Spinola SM; Characterization of a novel lipoprotein expressed by Haemophilus ducreyi.; Infect Immun; 1996 Dec; 64(12); 5047-5052

Anthony Campagnari, Ducey TF, Rebmann CA; Outer membrane protein B1, an iron-repressible protein conserved in the outer membrane of Moraxella (Branhamella) catarrhalis, binds human transferrin.; Infect Immun; 1996 Sep; 64(9); 3920-3924

Spinola SM, Orazi A, Arno JN, Fortney K, Kotylo P, Chen CY, Anthony Campagnari, Hood AF; Haemophilus ducreyi elicits a cutaneous infiltrate of CD4 cells during experimental human infection.; J Infect Dis; 1996 Feb; 173(2); 394-402

Melaugh W, Anthony Campagnari, Gibson BW; The lipooligosaccharides of Haemophilus ducreyi are highly sialylated.; J Bacteriol; 1996 Jan; 178(2); 564-570

Thomas Russo, Sharma G, Brown CR, Anthony Campagnari; Loss of the O4 antigen moiety from the lipopolysaccharide of an extraintestinal isolate of Escherichia coli has only minor effects on serum sensitivity and virulence in vivo.; Infect Immun; 1995 Apr; 63(4); 1263-1269

Anthony Campagnari, Shanks KL, Dyer DW; Growth of Moraxella catarrhalis with human transferrin and lactoferrin: expression of iron-repressible proteins without siderophore production.; Infect Immun; 1994 Nov; 62(11); 4909-4914

Melaugh W, Phillips NJ, Anthony Campagnari, Tullius MV, Gibson BW; Structure of the major oligosaccharide from the lipooligosaccharide of Haemophilus ducreyi strain 35000 and evidence for additional glycoforms.; Biochemistry; 1994 Nov; 33(44); 13070-13078

Westerink MA, Anthony Campagnari, Giardina P, Apicella MA; Antiidiotype antibodies as surrogates for polysaccharide vaccines.; Ann N Y Acad Sci; 1994 Aug; 730; 209-216

Anthony Campagnari, Karalus R, Apicella M, Melaugh W, Alan Lesse, Gibson BW; Use of pyocin to select a Haemophilus ducreyi variant defective in lipooligosaccharide biosynthesis.; Infect Immun; 1994 Jun; 62(6); 2379-2386

Spinola SM, Linda Wild, Apicella MA, Gaspari AA, Anthony Campagnari; Experimental human infection with Haemophilus ducreyi.; J Infect Dis; 1994 May; 169(5); 1146-1150

Brentjens RJ, Spinola SM, Anthony Campagnari; Haemophilus ducreyi adheres to human keratinocytes.; Microb Pathog; 1994 Mar; 16(3); 243-247

Gibson BW, Melaugh W, Phillips NJ, Apicella MA, Anthony Campagnari, Griffiss JM; Investigation of the structural heterogeneity of lipooligosaccharides from pathogenic Haemophilus and Neisseria species and of R-type lipopolysaccharides from Salmonella typhimurium by electrospray mass spectrometry.; J Bacteriol; 1993 May; 175(9); 2702-2712

Melaugh W, Phillips NJ, Anthony Campagnari, Karalus R, Gibson BW; Partial characterization of the major lipooligosaccharide from a strain of Haemophilus ducreyi, the causative agent of chancroid, a genital ulcer disease.; J Biol Chem; 1992 Jul; 267(19); 13434-13439

Sarwar J, Anthony Campagnari, Kirkham C, Murphy TF; Characterization of an antigenically conserved heat-modifiable major outer membrane protein of Branhamella catarrhalis.; Infect Immun; 1992 Mar; 60(3); 804-809

Anthony Campagnari, Linda Wild, Griffiths GE, Karalus RJ, Wirth MA, Spinola SM; Role of lipooligosaccharides in experimental dermal lesions caused by Haemophilus ducreyi.; Infect Immun; 1991 Aug; 59(8); 2601-2608

Haase EM, Anthony Campagnari, Sarwar J, Shero M, Wirth M, Cumming CU, Murphy TF; Strain-specific and immunodominant surface epitopes of the P2 porin protein of nontypeable Haemophilus influenzae.; Infect Immun; 1991 Apr; 59(4); 1278-1284

Spinola SM, Kwaik YA, Alan Lesse, Anthony Campagnari, Apicella MA; Cloning and expression in Escherichia coli of a Haemophilus influenzae type b lipooligosaccharide synthesis gene(s) that encodes a 2-keto-3-deoxyoctulosonic acid epitope.; Infect Immun; 1990 Jun; 58(6); 1558-1564

Westerink MA, Giardina PC, Anthony Campagnari, Apicella MA; The thymus-dependent nature of the murine antibody response to a monoclonal anti-idiotypic antibody to the Neisseria meningitidis serogroup C capsular polysaccharide.; Microb Pathog; 1990 Jun; 8(6); 411-419

Anthony Campagnari, Spinola SM, Alan Lesse, Kwaik YA, Mandrell RE, Apicella MA; Lipooligosaccharide epitopes shared among gram-negative non-enteric mucosal pathogens.; Microb Pathog; 1990 May; 8(5); 353-362

Alan Lesse, Anthony Campagnari, Bittner WE, Apicella MA; Increased resolution of lipopolysaccharides and lipooligosaccharides utilizing tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis.; J Immunol Methods; 1990 Jan; 126(1); 109-117

Fukatsu A, Yuzawa Y, Olson L, Miller J, Milgrom M, Zamlauski-Tucker MJ, Van Liew JB, Anthony Campagnari, Niesen N, Patel J; Interaction of antibodies with human glomerular epithelial cells.; Lab Invest; 1989 Oct; 61(4); 389-403

Westerink MA, Anthony Campagnari, Nelson MB, Murphy TF, Apicella MA; New concepts in vaccines for mucosal non-enteric human bacterial pathogens.; Adv Exp Med Biol; 1989; 251; 17-31

Murphy TF, Anthony Campagnari, Nelson MB, Apicella MA; Somatic antigens of Haemophilus influenzae as vaccine components.; Pediatr Infect Dis J; 1989 Jan; 8(1 Sup); 66-68

Westerink MA, Anthony Campagnari, Wirth MA, Apicella MA; Development and characterization of an anti-idiotype antibody to the capsular polysaccharide of Neisseria meningitidis serogroup C.; Infect Immun; 1988 May; 56(5); 1120-1127

Murphy TF, Bernstein JM, Dryja DM, Anthony Campagnari, Apicella MA; Outer membrane protein and lipooligosaccharide analysis of paired nasopharyngeal and middle ear isolates in otitis media due to nontypable Haemophilus influenzae: pathogenetic and epidemiological observations.; J Infect Dis; 1987 Nov; 156(5); 723-731

Campagnari AA, Gupta MR, Dudas KC, Murphy TF, Apicella MA; Antigenic diversity of lipooligosaccharides of nontypable Haemophilus influenzae.; Infect Immun; 1987 Apr; 55(4); 882-887

Murphy TF, Bartos LC, Anthony Campagnari, Nelson MB, Apicella MA; Antigenic characterization of the P6 protein of nontypable Haemophilus influenzae.; Infect Immun; 1986 Dec; 54(3); 774-779

Harvey, S., Douglass, H.O., Holyoke, E.D. and Goldrosen, M.H.; Localization of an acidic protein isolated from the effusion fluid of patients with pancreatic cancer.; Tumor Diagnostik and Therapie.; 1986; 7; 5-9

Apicella MA, Dudas KC, Anthony Campagnari, Rice P, Joseph Mylotte, Murphy TF; Antigenic heterogeneity of lipid A of Haemophilus influenzae.; Infect Immun; 1985 Oct; 50(1); 9-14

Harvey, S., Douglass, H.O., Holyoke, E.D. and Goldrosen, M.H.; Detection, isolation and biochemical characterization of an acidic protein from the effusion fluid of patients with pancreatic cancer.; Tumor Diagnostik and Therapie.; 1985; 6; 207-214

Goldrosen MH, Anthony Campagnari, Howell JH, Harvey S, Jenkins D, Berjian R, Harold Douglass; Immunodiagnosis of pancreatic cancer.; Prog Clin Biol Res; 1983; 133; 167-182

GRANTS

November 2002 to November 2005 Comprehensive Analysis of Virulence Factors and Potential Vaccine Antigens of Haemophilus ducreyi. The John R. Oishei Foundation Anthony Campagnari $300,000

September 2002 to September 2007 Genetics & Biology of M. catarrhalis LOS in Otitis Media NIDCD Anthony Campagnari $1,725,060

June 2002 to May 2004 Capture, Isolation and Identification of Biologic Agents New York State Center for Applied Technology Anthony Campagnari $428,000

August 2001 to July 2005 Analysis of Moraxella catarrhalis LOS: Role in Immunity National Institute of Allergy and Infectious Diseases Anthony Campagnari $851,269

June 2001 to May 2002 Development of Monoclonal Antibodies University Service Account Anthony Campagnari $59,108

February 2000 to January 2005 Analysis of M. catarrhalis receptors as Vaccine Antigens National Institute of Allergy and Infectious Diseases Anthony Campagnari $906,722

June 1997 to May 2002 Structure of Haemophilus ducreyi LOS: Role in Adherence/Invasion National Institute of Allergy and Infectious Diseases Anthony Campagnari $806,769