Dr. Arlene Collins
Microbiology and Immunology
218 Sherman Hall
Buffalo NY, 14214
Phone: (716) 829-2161
Email: acollins@buffalo.edu

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DESCRIPTION OF INTERESTS

In the area of research, I have selected the coronaviruses as a field of study. My research has lead to the identification of the coronavirus "Spike" glycoprotein as the attachment protein and found the major histocompatibility antigen, class I which plays a role in immune recognition, as a cell entry co-factor for the virus. Several of my publications have been repeatedly cited in textbooks and review articles. I have also demonstrated a direct inhibitory effect of cystatin C on coronavirus cysteine proteases. My goals are to identify the factors that participate in pathogenesis of the human coronaviruses and evaluation of the need for a vaccine. Currently, my investsigation of the role of innate immune responses to coronaviruses id directed toward identifying the molecular basis of inhibition of interferon induction by certain human coronavirus OC43 strains.
In the area of teaching I have taken advantage of the new trend in medical school teacing by participating in the Scientific Basis on medicine course by contributing teaching materials on AIDS and as a facilitator in order to broaden my teaching skills. In addition, I participate in the Developmental Neuroscience Microbiology Honors program for Medical Students.
In the area of service, I am an ad hoc reviewer for many journals and have served on two scientific review panels this year.


EDUCATION

1967 Ph.D., State University of New York at Buffalo
1964 Master of Arts, State University of New York at Buffalo
1961 Bachelor of Arts, D'Youville College


PUBLICATIONS

Collins AR, Yeh, E.A., Cohen, M.E., Faden, H.; Detection of coronavirus in the central nervous system of a child with acute disseminated encephalomyelitis; Pediatrics; 2004; 113; 73-76
Collins A; In Vitro Detection of Apoptosis in Monocytes/Macrophages Infected with Human Coronaviruses; Clinical and Diagnostic Laboratory Immunology; 2002 Nov; 9(6); 1392-1395
Yuan L, Collins AR, Dai J, Dubocovich ML, Hill SM; MT(1) melatonin receptor overexpression enhances the growth suppressive effect of melatonin in human breast cancer cells.; Mol Cell Endocrinol; 2002 Jun; 192(1-2); 147-156
Arlene Collins; Viral Effects on Neurons; Basic concepts in Neuroscience; 2002 Jan;
Arlene Collins; Induction of Apoptosis in MRC-5, diploid human fetal lung cells after infection with human coronavirus OC43; Adv. Exptl. Med. and Biol.; 2001 Jan; 494; 677-682
Collins AR, Grubb A; Cystatin D, a natural salivary cysteine protease inhibitor, inhibits coronavirus replication at its physiologic concentration.; Oral Microbiol Immunol; 1998 Feb; 13(1); 59-61
Arlene Collins; Human Macrophages are susceptible to coronavirus OC43. in corona and arteriviruses; Adv. Exptl. Med. and Biol.; 1998 Jan; 440; 635-639
Arlene Collins; Monoclonal Antibodies; The World Book Encyclopedia CD-ROM; 1997 Jan;
Collins AR; Interferon gamma potentiates human coronavirus OC43 infection of neuronal cells by modulation of HLA class I expression.; Immunol Invest; 1995 Nov; 24(6); 977-986
Arlene Collins; Identification of 120kD and 30kD receptors for human coronavirus OC43 in cell membrane preparations from newborn mice; Adv. Exptl. Med. and Biol.; 1995 Jan; 380; 387-390
Gu, M, Haraszthy, G., Arlene Collins, Bergey, E.J.; Identification of constituenets which inhibit herpes simplex virus 1 replication; Oral Microbiol. Immunol.; 1995 Jan; 10; 54-59
Collins AR; Human coronavirus OC43 interacts with major histocompatibility complex class I molecules at the cell surface to establish infection.; Immunol Invest; 1994 Aug; 23(4-5); 313-321
Arlene Collins; Virus-ligand interactions of OC43 coronavirus with cell membranes; Coronaviruses; 1994 Jan;
Bergey EJ, Gu M, Collins AR, Bradway SD, Levine MJ; Modulation of herpes simplex virus type 1 replication by human salivary secretions.; Oral Microbiol Immunol; 1993 Apr; 8(2); 89-93
Collins AR; HLA class I antigen serves as a receptor for human coronavirus OC43.; Immunol Invest; 1993 Mar; 22(2); 95-103
Arlene Collins; Virus-Ligand interactions of OC43 coronavirus with cell membranes; Adv. Exptl. Med. and Biol.; 1993 Jan; 334; 285-291
Collins AR, Grubb A; Inhibitory effects of recombinant human cystatin C on human coronaviruses.; Antimicrob Agents Chemother; 1991 Nov; 35(11); 2444-2446
Arlene Collins; Comparison of the replication of distinct strains of human coronavirus OC43 in organotypic human colon cells (Caco-2) and in mouse intestine; Adv. Exptl. Biol. Med.; 1990 Jan; 276; 491-496
Arlene Collins; Immunogenic Characteristics of Microbial Components and Products: Arenaviruses; Microbial Antigenodiagnosis; 1987 Jan;
Arlene Collins; Monoclonal Antibodies; The World Book Encyclopedia; 1987 Jan;
Arlene Collins; Regulation of persistent infections with human coronavirus OC43; Adv. Exptl. Biol. Med.; 1987 Jan; 218; 273-274
Collins AR, Sorensen O; Regulation of viral persistence in human glioblastoma and rhabdomyosarcoma cells infected with coronavirus OC43.; Microb Pathog; 1986 Dec; 1(6); 573-582
Sorensen, O., Arlene Collins, Flintoff, W., Ebers, G., Dales, S.; Probing for the human coronavirus OC43 in CNS tissue from multiple sclerosis patients; Neurology; 1986 Jan; 36; 1604-1606
Collins AR, Tunison LA, Knobler RL; Mouse hepatitis virus type 4 infection of primary glial cultures from genetically susceptible and resistant mice.; Infect Immun; 1983 Jun; 40(3); 1192-1197
Arlene Collins, Buchmeier, M. J.; Monoclonal Antibodies to Murine Hepatitis Virus-4 (JHM strain) show that the Viral Peplomer contains separate sites for attachment and cell-cell fusion; Immunol. Comm.; 1983 Jan; 12; 135
Collins AR, Knobler RL, Powell H, Buchmeier MJ; Monoclonal antibodies to murine hepatitis virus-4 (strain JHM) define the viral glycoprotein responsible for attachment and cell--cell fusion.; Virology; 1982 Jun; 119(2); 358-371
Arlene Collins; Orthomyxoviruses and Paramyxoviruses; Medical Microbiology; 1982 Jan;
Arlene Collins; Picornaviruses; Medical Microbiology; 1982 Jan;
Arlene Collins; Poxviruses and Adenoviruses; Medical Microbiology; 1982 Jan;
Arlene Collins; Arenaviruses: Marburg and Ebola Agents; Principles of Immunological Diagnosis in Medicine; 1981 Jan;
Arlene Collins, Flanagan, T. D.; Cloning and Antibody Studies of a Variant of Sendal Virus with Temperature Sensitive Hemolytic Activity; J. Supra-molecular Structure; 1978 Jan; 8(2); 263
Arlene Collins, Flanagan, T. D.; Recovery of a Sendai Virus Variant with Temperature-Sensitive Hemolytic Activity from Persistently Infected Cells from Mouse Brain; Arch. Virol.; 1978 Jan; 58; 81-93
Arlene Collins, Flanagan, T. D.; Interferon Production and Response to Exogenous Interferon in Two Cell Lines of mouse Brain Origin Persistently Infected with Sendai Virus; Arch. Virol.; 1977 Jan; 53; 313-321
Menna JH, Collins AR, Flanagan TD; Characterization of an in vitro persistent-state measles virus infection: establishment and virological characterization of the BGM/MV cell line.; Infect Immun; 1975 Jan; 11(1); 152-158
Menna JH, Collins AR, Flanagan TD; Characterization of an in vitro persistent-state measles virus infection: species characterization and interference in the BGM/MV cell line.; Infect Immun; 1975 Jan; 11(1); 159-163
Collins AR, Barron AL; Demonstration of group- and species-specific antigens of chlamydial agents by gel diffusion.; J Infect Dis; 1970 Jan; 121(1); 1-8
Barron AL, Collins AR; Studies on trachoma agent by double diffusion gel precipitation.; Am J Ophthalmol; 1967 May; 63(5); 1487-1491
Collins AR, Barron AL, Shulman S; Comparison of bouyant density of two mutants of ECHO 6 by density gradient ultracentrifugation.; Proc Soc Exp Biol Med; 1967 Mar; 124(3); 850-855
Collins A; In vitro Detection of Apoptosis in Monocyte/Macrophages Infected with Human Coronaviruses; Clinical and Diagnostic Laboratory Immunology; 9(6); 1392-1395


GRANTS

August 2001 to August 2003
RT-PCR Analysis for Coronaviruses in Neuropsychiatric Diseases
The Stanley Foundation
Arlene Collins
$100,000