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OFFICE LOCATIONS
Erie County Medical Center
462 Grider St.
Buffalo NY, 14215
Phone: 716-898-4759
Fax: 716-829-3889
 View map |
Insurance Accepted
Better Health Plan, Inc. (HMO)
Community Blue (HMO)
Empire
Fidelis
GHI
Independent Health Association (HMO)
United Healthcare
Univera Health Care (HMO) |
DESCRIPTION OF INTERESTS
Microbial toxins
Enteric infectious diseases
Enteropathogenic E. coli
Cellular mechanisms of diarrhea
My research is on the pathogenesis of diarrhea due to enterotoxigenic Escherichia coli (ETEC) and enteropathogenic Escherichia coli (EPEC). ETEC and EPEC are important causes of diarrhea among children in developing countries. Regarding ETEC, my research has focussed on the mechanism of action of the heat-stable enterotoxin, STa, which acts by stimulating membrane-bound guanylyl cyclase in intestinal cells, generating cyclic GMP.
My laboratory‘s interest in EPEC has focussed on the activation of protein kinase pathways within the host cell, and on the ability of EPEC to trigger death of the host cell. We have recently shown that EPEC-induced cell death shows features of apoptosis, or programmed cell death. |
SPECIALTIES
EDUCATION
| 1990 |
Fellowship, Infectious Diseases
University of Virginia
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| 1987 |
Residency, Medicine
University of Rochester, Strong Memorial Hospital
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| 1984 |
M.D., MD
University of Virginia
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| 1983 |
Ph.D., Pharmacology
University of Virginia
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EXPERTISE
PUBLICATIONS
| Crane JK, Choudhari SS, Naeher TM, Duffey ME; Mutual enhancement of virulence by enterotoxigenic and enteropathogenic Escherichia coli.; Infect Immun; 2006 Mar; 74(3); 1505-1515 |
| CRANE JK; Externalization of host cell protein kinase C during enteropathogenic Escherichia coli infection.; Cell Death and Differentiation; 2003; |
| CRANE JK; Pulmonary mucormycosis in a patient with COPD; diagnosis by fine-needle aspiration cytology.; Journal of Thoracic and Cardiovascular Surgery; 2003; |
| Crane JK, Olson RA, Jones HM, Duffey ME; Release of ATP during host cell killing by enteropathogenic E. coli and its role as a secretory mediator.; Am J Physiol Gastrointest Liver Physiol; 2002 Jul; 283(1); 74-86 |
| Crane JK, McNamara BP, Donnenberg MS; Role of EspF in host cell death induced by enteropathogenic Escherichia coli.; Cell Microbiol; 2001 Apr; 3(4); 197-211 |
| Crane JK; Redistribution of cyclic GMP in response to sodium butyrate in colon cells.; Arch Biochem Biophys; 2000 Apr; 376(1); 163-170 |
| Crane JK; Preformed bacterial toxins.; Clin Lab Med; 1999 Sep; 19(3); 583-599 |
| Aljada IS, Crane JK, Corriere N, Wagle DG, Amsterdam D; Mycobacterium bovis BCG causing vertebral osteomyelitis (Pott's disease) following intravesical BCG therapy.; J Clin Microbiol; 1999 Jun; 37(6); 2106-2108 |
| Crane JK, Majumdar S, Pickhardt DF; Host cell death due to enteropathogenic Escherichia coli has features of apoptosis.; Infect Immun; 1999 May; 67(5); 2575-2584 |
| Kotlarz JP, Crane JK; Toxic shock syndrome after mastoidectomy.; Otolaryngol Head Neck Surg; 1998 May; 118(5); 701-702 |
| Crane JK, Oh JS; Activation of host cell protein kinase C by enteropathogenic Escherichia coli.; Infect Immun; 1997 Aug; 65(8); 3277-3285 |
| Crane JK, Shanks KL; Phosphorylation and activation of the intestinal guanylyl cyclase receptor for Escherichia coli heat-stable toxin by protein kinase C.; Mol Cell Biochem; 1996 Dec; 165(2); 111-120 |
| Crane JK, Shih HT; Syncope and cardiac arrhythmia due to an interaction between itraconazole and terfenadine.; Am J Med; 1993 Oct; 95(4); 445-446 |
| Okhuysen PC, Crane JK, Pappas J; St. Louis encephalitis in patients with human immunodeficiency virus infection.; Clin Infect Dis; 1993 Jul; 17(1); 140-141 |
| Crane JK, Wehner MS, Bolen EJ, Sando JJ, Linden J, Guerrant RL, Sears CL; Regulation of intestinal guanylate cyclase by the heat-stable enterotoxin of Escherichia coli (STa) and protein kinase C.; Infect Immun; 1992 Dec; 60(12); 5004-5012 |
| Levine SA, Donowitz M, Watson AJ, Sharp GW, Crane JK, Weikel CS; Characterization of the synergistic interaction of Escherichia coli heat-stable toxin and carbachol.; Am J Physiol; 1991 Oct; 261(4 Pt); 592-601 |
| Tunkel AR, Crane JK, Hayden FG; Pulmonary nocardiosis in AIDS.; Chest; 1991 Jul; 100(1); 295-296 |
| Crane JK, Burrell LL, Weikel CS, Guerrant RL; Carbachol mimics phorbol esters in its ability to enhance cyclic GMP production by STa, the heat-stable toxin of Escherichia coli.; FEBS Lett; 1990 Nov; 274(1-2); 199-202 |
| Weikel CS, Spann CL, Chambers CP, Crane JK, Linden J, Hewlett EL; Phorbol esters enhance the cyclic GMP response of T84 cells to the heat-stable enterotoxin of Escherichia coli (STa).; Infect Immun; 1990 May; 58(5); 1402-1407 |
| Crane JK, Hewlett EL, Weikel CS; Failure of pertussis toxin to inhibit activation of guanylate cyclase by the heat-stable enterotoxin of Escherichia coli (STa) in the T84 cell line.; Infect Immun; 1989 Apr; 57(4); 1186-1191 |
| Campanile CP, Crane JK, Peach MJ, Garrison JC; The hepatic angiotensin II receptor. I. Characterization of the membrane-binding site and correlation with physiological response in hepatocytes.; J Biol Chem; 1982 May; 257(9); 4951-4958 |
| Crane JK, Campanile CP, Garrison JC; The hepatic angiotensin II receptor. II. Effect of guanine nucleotides and interaction with cyclic AMP production.; J Biol Chem; 1982 May; 257(9); 4959-4965 |
GRANTS
September 2003 to August 2005
Enteropathogenic and toxigenic E. coli Interactions
NIH
John Crane
Michael E. Duffey
$225,020
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September 2003 to January 2006
Host cell killing by EPEC: central role in pathogenesis
NIH, NIAID
John K. Crane
$405,000
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