Dr. Suzanne Laychock Pharmacology And Toxicology 134 Farber Hall Buffalo NY, 14214 Phone: (716) 829-2808 Email: laychock@buffalo.edu View map

DESCRIPTION OF INTERESTS

Endocrine pharmacology with an emphasis on signal transduction mechanisms involved in secretion is the focus of research in this laboratory. Our research is primarily concerned with the regulation of insulin secretion from pancreatic islets of Langerhans. The islet of Langerhans is a minute pearl-shaped organ in the pancreas which is made up of thousands of endocrine cells which secrete either insulin, glucagon, or somatostatin. The pancreas itself has thousands of islets which secrete hormones to regulate blood glucose levels. The research focus emphasizes investigation of the cellular mechanisms which mediate insulin secretion in beta-cells of the islet. The role of second messengers, including cyclic nucleotides, inositol phosphates, diglyceride, calcium and eicosanoids is central to our understanding of the signal transduction mechanisms which control insulin secretion. Knowledge of the regulation of insulin secretion is paramount to our understanding the disease Diabetes Mellitus. Diabetes mellitus is characterized as having two major subdivisions: type I or insulin- dependent diabetes mellitus (IDDM) - previously called "juvenile diabetes" - occurs when the pancreatic beta cells of islets are destroyed and no insulin is available to promote glucose metabolism; type II or noninsulin- dependent diabetes mellitus (NIDDM) - previously called "adult onset diabetes" -occurs when either there is insulin resistance and insulin cannot evoke a normal physiological response, or when there is insufficient insulin secreted by the pancreatic islet beta-cell and glucose cannot be metabolized normally. In vitro systems have been developed in this lab which attempt to mimic certain aspects of either IDDM or NIDDM. The disease processes associated with IDDM include autoimmune destruction of the beta-cell. This outcome appears to be mediated by T-cells and macrophages which secrete cytokines in the vicinity of the beta-cells. Cytokines are cytotoxic by virtue of their initiation of events in cells which promote responses resulting in apoptosis and cell death. The beta-cell specifically in the islet is susceptible to destruction by the cytokines interleukin-1 beta (IL-1B), tumor necrosis factor alpha, and interferon gamma. Islets in primary culture are studied in this lab in order to allow us to evaluate the effects of cytokines on specific pathways and allow us to identify potential pharmacological agents which can interact in various ways with the cytokines and related activated pathways. Cytokines specifically induce nitric oxide synthase in beta-cells, which results in the production of reactive oxygen species, especially the free radical nitric oxide. Studies in this lab are focused on determining which pharmacologic agents interfere with the cytokine-initiated cell killing, and what their mechanism of action is in preventing cell destruction. Recent studies have investigated the role of certain steroids as protective agents in islets exposed to IL-1B. In addition, exciting studies are currently underway to determine the protective role of specific heavy metals in IL-1B-treated islets. These studies are examining the role of the promoter NF-kB, and nitric oxide synthase activation in cytokine and other pharmacologic agent actions. The ability of catalase, superoxide dismutase, heme oxygenase and perhaps other heat shock/stress proteins and protein kinase pathways to affect the cellular response to cytokines are also being investigated. In vitro primary islet cultures are also studied as a model of NIDDM. In NIDDM, islets are often characterized as "desensitized" to glucose stimulation, since the islets seem to become fatigued during continuous glucose stimulation during hyperglycemia and fail to respond normally in terms of insulin release. The result is low insulin secretion and diabetes mellitus. In the in vitro model, islets are cultured at elevated glucose levels which simulate diabetes mellitus. In this way, we are able to investigate the effects of short- and long-term glucose exposure on biochemical, molecular and physiological parameters related to insulin secretion. The model has been successful in helping us characterize the onset of "glucose desensitization" in terms of specific cell regulatory pathways, including the generation of cyclic AMP and ATP, the activity of the phosphoinositide pathway, and the regulation of cell calcium levels. Recent studies demonstrate that gene transcription as determined by reverse transcriptase-polymerase chain reaction mediates changes in the islet calcium regulatory pathways. In addition, calcium imaging by digital fluorescence spectroscopy has allowed us to directly quantitate changes in beta-cell calcium levels. Currently studies are underway to characterize the stress responses of islets. Stressors include temperature, cytokines, high and low glucose, and oxidative changes. Effectors include stress activated protein kinases (SAPKs), heme oxygenase (HO-1), heat shock proteins (hsp), superoxide dismutase and catalase, among others. Reactor systems include many of the second messenger pathways in addition to promoter elements and transcription factors. Identification of new participating processes in the beta-cell and responses to stress will enable us to better understand the disease diabetes mellitus and the future frontiers in the pharmacological management of the disease.

SPECIALTIES

Apoptosis and cell death Endocrinology Molecular and Cellular Biology Regulation of gene expression Regulation of metabolism Signal Transduction

EDUCATION

1976	Ph.D., Pharmacology Medical College of Virginia
1973	M.A., Biology City University of New York
1971	B.S., Biology Brooklyn College

EXPERTISE

gene regulation mRNA analysis, RT-PCR

metabolism glycolysis, glucose oxidation

secretion insulin secretion

signal transduction second messenger analysis,cyclic AMP,prostaglandins,calcium,lipids,phospholipids,cyclic GMP, inositol trisphosphate

PUBLICATIONS

You, H. and Laychock, S.G.; Atrial natriuretic peptide (ANP) and islet beta-cell growth; FASEB J; 2008;

Tian, J. Lei, P. Laychock, S.G. Andreadis, S.T.; Regulated Insulin Delivery from Human Epidermal Cells Reverses Hyperglycemia; Molecular Therapy; 2008; 16; 1146-1153

Lei, P., Ogunade, A., Kirkwood, K. L., Laychock, S. G., and Andreadis, S. T.; Efficient production of bioactive insulin from human epidermal keratinocytes and tissue engineered skin substitutes: implications for treatment of diabetes.; Tissue Engineering; 2007; 13; 2119-2131

Tian, J., Lei, P., Laychock, S.G., Andreadis, S.T.; Regulated secretion of insulin from genetically modified skin cells for treatment of diabetes; Biomedical Engineering Society Proceedings; 2007;

Tian, J., Lei, P., Laychock, S.G., Andreadis, S.T; Regulated secretion of insulin from genetically modified skin cells for treatment of diabetes.; Bio Med Eng Soc; 2007;

Laychock, S.G., Sessanna, S.M., Mastrandrea, L.D.; Sphingosine Kinase Expression and Activity in Rat Pancreatic Islets.; Diabetes; 2007; 56; A445

Laychock SG, Sessanna SM, Lin M-H, Mastrandrea LD; Sphingosine 1-phosphate affects cytokine-induced apoptosis in pancreatic islet beta-cells.; Endocrinology; 2006; 147; 4705-4712

Laychock, S.G., Sessanna, S.M.; Sphingosine 1-phosphate modulates apoptosis in cytokine challenged pancreatic islet β-cells.; FASEB J; 2006; 20; A347

Mastrandrea, L.D. Sessanna, S.M. Laychock, S.G. ; Sphingosine kinase in Pancreatic Islets is Activated by Glucose and Glucose Analogues; Diabetes; 2006; 55; A376

Mastrandrea, L.D., Sessanna, S.M., Laychock, S.G; Sphingosine kinase in Pancreatic Islets is Activated by Glucose and Glucose Analogues.; Diabetes; 2006; 55; A376

Laychock, S.G., Sessanna, S.M., Lin, M-H; Effects of sphingosine-1-phosphate (S1P) on cytokine-induced apoptosis in pancreatic islets and INS-1 cells.; Diabetes; 2005; 54(sup 1); A78

Patel, M.S., Srinivasan, M., Laychock, S.G.; Nutrient Induced Maternal Hyperinsulinemia and Metabolic Programming in the Progeny; ; 2005; 55; 137-152

Mastrandrea, L., Laychock, S.G.; Sphingosine kinase 2 is the predominant enzyme in rat pancreatic islets and INS-1 cells and is activated by cytokines.; FASEB J; 2005; 19; A527

Mastrandrea LD, Laychock SG; Sphingosine kinase activity and sphingosine-1-phosphate production in rat pancreatic islets and INS-1 cells: response to cytokines.; Diabetes 54:1429-1436; 2005; 54; 1429-1436

Mastrandrea L, Laychock SG; Characterization of sphingosine-1 phosphate phosphohydrolase activity in INS-1 Cells.; FASEB J; 2004; LB587;

Patel, MS, Srinivasan, M, Laychock SG; Nutrient-induced maternal hyperinsulinemia and metabolic programming in the progeny.; Nestle Nutr Workshop Ser Pediatr Program; 2004; 55; 137-147

Laychock SG; The Pancreas and Pancreatic Hormones.; X-Pharm; 2004;

Mastrandrea, L.D., Sessanna, S., Tian, Y. and Laychock, S.G.; EDG receptor and sphingosine-1-phosphate signaling in rat islets and INS-1 cells.; Diabetes; 2003; 52; A383

Laychock, S.G., Mastrandrea, L.D., Sessanna, S.M.; EDG receptors, sphingosine-1-phosphate and apoptosis.; EASD Islet Study Group Meeting; 2003;

Laychock S, Tian Y, Sessanna S; Endothelial differentiation gene (EDG) receptors in pancreatic islets and INS-1 cells; Diabetes; 2003; 52; 1986-1993

Laychock SG; Insulin Receptor Signaling.; Encyclopedia of Hormones; 2003;

Srinivasan, M., Hill, D.J., Laychock, S.G., Patel, M.S.; Neonatal nutrition: metabolic programming of pancreatic islets and obesity.; Experimental Biology & Medicine; 2003; 228; 15-23

Tian Y, and Laychock SG; Prolactin regulates adenylyl cyclase and insulin secretion in rat pancreatic islets.; Molecular and Cellular Endocrinology; 2003; 204; 75-84

Takeuchi, T., Aletta J, Laychock S, Tian, Y., Rubin R; Role of nerve growth factor in the regulation of parotid cell differentiation induced by rat serum; Biochem. Pharmacol. 65: 1507-1513 (2003); 2003;

Laychock, S.G., Sessanna, S.M., Tian, Y., and Mastrandrea, L.D.; Sphingosine-1-phosphate signaling and beta-cell survival.; Diabetologia; 2003; 46(sup 2); A187

Lee, B.S., Sessanna, S., Laychock, S.G., & Rubin, R.P.; Expression and Cellular Localization of a Modified Type 1 Ryanodine Receptor and L-Type Channel Proteins in Non-Muscle Cells; J. Membrane Biol. 189: 181-190 (2002); 2002; 189; 181-190

Srinivasan M, Laychock SG, Hill DJ, Patel MS; Neonatal Nutrition: Metabolic programming of pancreatic islets and obesity; Experimental Biology & Medicine; 2002; 228(1); 15-23

Tian Y, Laychock SG; Protein kinase C and calcium regulation of adenylyl cyclase in isolated rat pancreatic islets.; Diabetes; 2001 Nov; 50(11); 2505-2513

Lee B, Gai W, Laychock SG; Proteasomal activation mediates down-regulation of inositol 1,4,5-trisphosphate receptor and calcium mobilization in rat pancreatic islets.; Endocrinology; 2001 May; 142(5); 1744-1751

Lee B, Laychock SG; Inositol 1,4,5-trisphosphate receptor isoform expression in mouse pancreatic islets: effects of carbachol.; Biochem Pharmacol; 2001 Feb; 61(3); 327-336

Lee B, Srinivasan M, Aalinkeel R, Patel MS, Laychock SG; Mitochondrial-encoded gene regulation in rat pancreatic islets.; Metabolism; 2001 Feb; 50(2); 200-206

Srinivasan M, Aalinkeel R, Song F, Lee B, Laychock SG, Patel MS; Adaptive changes in insulin secretion by islets from neonatal rats raised on a high-carbohydrate formula.; Am J Physiol Endocrinol Metab; 2000 Dec; 279(6); 1347-1357

Laychock SG, Duzen J, Simpkins CO; Metallothionein induction in islets of Langerhans and insulinoma cells.; Mol Cell Endocrinol; 2000 Jul; 165(1-2); 179-187

Lee B, Laychock SG; Regulation of inositol trisphosphate receptor isoform expression in glucose-desensitized rat pancreatic islets: role of cyclic adenosine 3',5'-monophosphate and calcium.; Endocrinology; 2000 Apr; 141(4); 1394-1402

Aalinkeel R, Srinivasan M, Kalhan SC, Laychock SG, Patel MS; A dietary intervention (high carbohydrate) during the neonatal period causes islet dysfunction in rats.; Am J Physiol; 1999 Dec; 277(6 Pt); 1061-1069

Lee B, Jonas JC, Weir GC, Laychock SG; Glucose regulates expression of inositol 1,4,5-trisphosphate receptor isoforms in isolated rat pancreatic islets.; Endocrinology; 1999 May; 140(5); 2173-2182

Ye J, Laychock SG; A protective role for heme oxygenase expression in pancreatic islets exposed to interleukin-1beta.; Endocrinology; 1998 Oct; 139(10); 4155-4163

Lee B, Bradford PG, Laychock SG; Characterization of inositol 1,4,5-trisphosphate receptor isoform mRNA expression and regulation in rat pancreatic islets, RINm5F cells and betaHC9 cells.; J Mol Endocrinol; 1998 Aug; 21(1); 31-39

Laychock SG; Rat pancreatic islet and RINm5F cell responses to epiandrosterone, dehydroepiandrosterone and interleukin-1 beta.; Biochem Pharmacol; 1998 May; 55(9); 1453-1464

Laychock SG; Determination of NOS activity using cyclic-GMP formation.; Methods Mol Biol; 1998; 100; 93-100

Lee B, Laychock SG; Atrial natriuretic peptide and cyclic nucleotides affect glucose-induced Ca2+ responses in single pancreatic islet beta-cells: correlation with (Ca[2+] + Mg2+)-ATPase activity.; Diabetes; 1997 Aug; 46(8); 1312-1318

Laychock SG; Modulation of glyceraldehyde 3-phosphate dehydrogenase activity in isolated pancreatic islets.; Biochem Pharmacol; 1996 Sep; 52(5); 793-799

Laychock SG, Bauer AL; Epiandrosterone and dehydroepiandrosterone affect glucose oxidation and interleukin-1 beta effects in pancreatic islets.; Endocrinology; 1996 Aug; 137(8); 3375-3385

Laychock SG, Vadlamudi S, Patel MS; Neonatal rat dietary carbohydrate affects pancreatic islet insulin secretion in adults and progeny.; Am J Physiol; 1995 Oct; 269(4 Pt); 739-744

Laychock SG; Impaired cyclic AMP response to stimuli in glucose-desensitized rat pancreatic islets.; Mol Cell Endocrinol; 1995 Aug; 113(1); 19-28

Desole MS, Kim WK, Rabin RA, Laychock SG; Nitric oxide reduces depolarization-induced calcium influx in PC12 cells by a cyclic GMP-mediated mechanism.; Neuropharmacology; 1994 Feb; 33(2); 193-198

Chang MC, Berkery D, Schuel R, Laychock SG, Zimmerman AM, Zimmerman S, Schuel H; Evidence for a cannabinoid receptor in sea urchin sperm and its role in blockade of the acrosome reaction.; Mol Reprod Dev; 1993 Dec; 36(4); 507-516

Xia M, Laychock SG; Insulin secretion, myo-inositol transport, and Na(+)-K(+)-ATPase in glucose-desensitized rat islets.; Diabetes; 1993 Oct; 42(10); 1392-1400

Laychock SG; Sp-5,6-dichloro-1-beta-D-ribofuranosylbenzimidazole-3',5'-cyclic monophosphorothioate is a potent stimulus for insulin release.; Endocr Res; 1993; 19(2-3); 113-122

Khalaf LJ, Laychock SG; Phosphatidylinositol availability and polyphosphoinositide synthesis in pancreatic islet cell membranes.; Biochem Pharmacol; 1992 Mar; 43(6); 1303-1310

Laychock SG, Modica ME, Cavanaugh CT; L-arginine stimulates cyclic guanosine 3',5'-monophosphate formation in rat islets of Langerhans and RINm5F insulinoma cells: evidence for L-arginine:nitric oxide synthase.; Endocrinology; 1991 Dec; 129(6); 3043-3052

Conway BR, Laychock SG, Rubin RP; Comparative effects of epidermal growth factor and carbachol on phosphoinositide synthesis and breakdown in pancreatic acinar cells.; Biochem Biophys Res Commun; 1991 Jul; 178(2); 780-785

Chang MC, Berkery D, Laychock SG, Schuel H; Reduction of the fertilizing capacity of sea urchin sperm by cannabinoids derived from marihuana. III. Activation of phospholipase A2 in sperm homogenate by delta 9-tetrahydrocannabinol.; Biochem Pharmacol; 1991 Jul; 42(4); 899-904

Rubin RP, Withiam-Leitch M, Laychock SG; Modulation of phospholipase A2 activity in zymogen granule membranes by GTP[S]; evidence for GTP-binding protein regulation.; Biochem Biophys Res Commun; 1991 May; 177(1); 22-26

Rubin RP, Thompson RH, Laychock SG; Characterization of phospholipase A2 and acyltransferase activities in purified zymogen granule membranes.; Biochim Biophys Acta; 1990 Aug; 1045(3); 245-251

Laychock SG; Fatty acids and cyclooxygenase and lipoxygenase pathway inhibitors modulate inositol phosphate formation in pancreatic islets.; Mol Pharmacol; 1990 Jun; 37(6); 928-936

Chaudhry A, Conway BR, Laychock SG, Rubin RP; Analysis of the regulation of phosphatidylinositol-4,5-bisphosphate synthesis by arachidonic acid in exocrine pancreas.; Arch Biochem Biophys; 1989 Aug; 272(2); 488-495

Laychock SG, Bilgin S; Calcium mobilization, prostaglandin E2 and alpha 2-adrenoceptor modulation of glucose utilization and insulin secretion in pancreatic islets.; Biochem Pharmacol; 1989 Aug; 38(15); 2511-2520

Laychock SG; Prostaglandin E2 inhibits phosphoinositide metabolism in isolated pancreatic islets.; Biochem J; 1989 May; 260(1); 291-294

Laychock SG; Prostaglandin E2 and alpha 2 adrenoceptor agonists inhibit the pentose phosphate shunt in pancreatic islets.; Arch Biochem Biophys; 1989 Feb; 269(1); 354-358

Hoffman JM, Laychock SG; Choline turnover in phosphatidylcholine of pancreatic islets. Implications for CDP-choline pathway.; Diabetes; 1988 Nov; 37(11); 1489-1498

Chaudhry A, Thompson RH, Rubin RP, Laychock SG; Relationship between delta-9-tetrahydrocannabinol-induced arachidonic acid release and secretagogue-evoked phosphoinositide breakdown and Ca2+ mobilization of exocrine pancreas.; Mol Pharmacol; 1988 Oct; 34(4); 543-548

Morgan RO, Laychock SG; Biosynthesis of peptidyl leukotrienes and other lipoxygenase products by rat pancreatic islets. Comparison with macrophages and neutrophils.; Prostaglandins; 1988 Apr; 35(4); 609-623

Chaudhry A, Laychock SG, Rubin RP; The effects of fatty acids on phosphoinositide synthesis and myo-inositol accumulation in exocrine pancreas.; J Biol Chem; 1987 Dec; 262(36); 17426-17431

Laychock SG; Alpha 2-adrenoceptor stimulation affects total glucose utilization in isolated islets of Langerhans.; Mol Pharmacol; 1987 Aug; 32(1); 241-248

Laychock SG, Bilgin S; Alpha 2-adrenergic inhibition of pancreatic islet glucose utilization is mediated by an inhibitory guanine nucleotide regulatory protein.; FEBS Lett; 1987 Jun; 218(1); 7-10

Laychock SG; Effects of guanosine 3',5'-monophosphate on glucose utilization in isolated islets of Langerhans.; Endocrinology; 1987 Feb; 120(2); 517-524

Laychock SG, Hoffman JM, Meisel E, Bilgin S; Pancreatic islet arachidonic acid turnover and metabolism and insulin release in response to delta-9-tetrahydrocannabinol.; Biochem Pharmacol; 1986 Jun; 35(12); 2003-2008

Laychock SG; Effects of hydroxyeicosatetraenoic acids on fatty acid esterification in phospholipids and insulin secretion in pancreatic islets.; Endocrinology; 1985 Sep; 117(3); 1011-1019

Laychock SG; Phosphatidylethanolamine N-methylation and insulin release in isolated pancreatic islets of the rat.; Mol Pharmacol; 1985 Jan; 27(1); 66-73

Kelly KL, Laychock SG; Activity of prostaglandin biosynthetic pathways in rat pancreatic islets.; Prostaglandins; 1984 Jun; 27(6); 925-938

Laychock SG; Identification and metabolism of polyphosphoinositides in isolated islets of Langerhans.; Biochem J; 1983 Oct; 216(1); 101-106

Laychock SG; Mediation of insulin release by cGMP and cAMP in a starved animal model.; Mol Cell Endocrinol; 1983 Oct; 32(2-3); 157-170

Laychock SG; Fatty acid incorporation into phospholipids of isolated pancreatic islets of the rat. Relationship to insulin release.; Diabetes; 1983 Jan; 32(1); 6-13

Rubin RP, Kelly KL, Halenda SP, Laychock SG; Arachidonic acid metabolism in rat pancreatic acinar cells: calcium-mediated stimulation of the lipoxygenase system.; Prostaglandins; 1982 Aug; 24(2); 179-193

Laychock SG; Phospholipase A2 activity in pancreatic islets is calcium-dependent and stimulated by glucose.; Cell Calcium; 1982 Mar; 3(1); 43-54

Garcia R, Laychock SG, Rubin RP; Inhibition of dibutyryl cyclic AMP induced steroidogenesis in rat adrenocortical cells by the putative calcium antagonist TMB-8.; J Steroid Biochem; 1982 Feb; 16(2); 317-322

Kelly KL, Laychock SG; Prostaglandin synthesis and metabolism in isolated pancreatic islets of the rat.; Prostaglandins; 1981 May; 21(5); 759-769

Laychock SG; Evidence for guanosine 3',5'-monophosphate as a putative mediator of insulin secretion from isolated rat islets.; Endocrinology; 1981 Apr; 108(4); 1197-1205

Laychock SG, Walker L; Evidence for 6-keto-PGF1 alpha in adrenal cortex of the rat and effects of 6-keto-PGF1 alpha and PGI2 on adrenal cAMP levels and steroidogenesis.; Prostaglandins; 1979 Nov; 18(5); 793-811

Laychock SG, Harada E, Rubin RP; Isoproterenol and nucleotide induced stimulation of Ca2+ uptake by microsomal fractions from kidney and isolated glomeruli.; Biochem Pharmacol; 1979 Nov; 28(21); 3205-3211

Laychock SG, Landon EJ, Hardman JG; The effect of adrenocorticotropin and nucleotides on Ca2+ uptake in adrenal cortical microsomal vesicles.; Endocrinology; 1978 Dec; 103(6); 2198-2206

Laychock SG, Hardman JG; Effects of sodium nitroprusside and ascorbic acid on rat adrenocortical cell cGMP levels and steroidogenesis.; J Cyclic Nucleotide Res; 1978 Oct; 4(5); 335-344

Harada E, Laychock SG, Rubin RP; Parathyroid hormone induced stimulation of calcium uptake by renal microsomes.; Biochem Biophys Res Commun; 1978 Sep; 84(2); 396-402

Rubin RP, Laychock SG; Prostaglandins and calcium-membrane interactions in secretory glands.; Ann N Y Acad Sci; 1978 Apr; 307; 377-390

Laychock SG, Shen JC, Carmines EL, Rubin RP; The effect of corticotropin on phospholipid metabolism in isolated adrenocortical cells.; Biochim Biophys Acta; 1978 Mar; 528(3); 355-363

Laychock SG, Franson RC, Weglicki WB, Rubin RP; Identification and partial characterization of phospholipases in isolated adrenocortical cells. The effects of synacthen [corticotropin-(1--24)-tetracosapeptide] and calcium ions.; Biochem J; 1977 Jun; 164(3); 753-756

Laychock SG, Rubin RP; Regulation of steroidogenesis and prostaglandin formation in isolated adrenocortical cells: the effects of pregnenolone and cycloheximide.; J Steroid Biochem; 1977 Jun; 8(6); 663-667

Rubin RP, Laychock SG, End DW; On the role of cyclic AMP and cyclic GMP in steroid production by bovine cortical cells.; Biochim Biophys Acta; 1977 Feb; 496(2); 329-338

Laychock SG, Warner W, Rubin RP; Further studies on the mechanisms controlling prostaglandin biosynthesis in the cat adrenal cortex: the role of calcium and cyclic AMP.; Endocrinology; 1977 Jan; 100(1); 74-81

Gemmell RT, Laychock SG, Rubin RP; Ultrastructural and biochemical evidence for a steroid-containing secretory organelle in the perfused cat adrenal gland.; J Cell Biol; 1977 Jan; 72(1); 209-215

Laychock SG, Rubin RP; Indomethacin-induced alterations in corticosteroid and prostaglandin release by isolated adrenocortical cells of the cat.; Br J Pharmacol; 1976 Jun; 57(2); 273-278

Laychock SG, Rubin RP; Radioimmunoassay measurement of ACTH-facilitated PGE2 and PGF2alpha release from isolated cat adrenocortical cells.; Prostaglandins; 1976 Apr; 11(4); 753-767

Laychock SG, Rubin RP; ACTH-induced prostaglandin biosynthesis from 3H-arachidonic acid by adrenocortical cells.; Prostaglandins; 1975 Sep; 10(3); 529-540

Rubin RP, Sheid B, McCauley R, Laychock SG; ACTH-induced protein release from the perfused cat adrenal gland: evidence for exocytosis?; Endocrinology; 1974 Aug; 95(2); 370-378

Laychock SG, Rubin RP; Isolation of ACTH-induced protein from adrenal perfusate.; Steroids; 1974 Aug; 24(2); 177-184

Hui You, Suzanne G Laychock; Atrial natriuretic peptide (ANP) and islet beta-cell growth; ExBio 2008;

Laychock SG; Coordinate interactions of cyclic nucleotide and phospholipid metabolizing pathways in calcium-dependent cellular processes.; Curr Top Cell Regul; 30; 203-242

Laychock SG; Glucose metabolism, second messengers and insulin secretion.; Life Sci; 47(25); 2307-2316

Laychock SG; The biochemistry of cell activation as related to the putative actions of flavonoids.; Prog Clin Biol Res; 213; 215-229

GRANTS

January 2006 to December 2007 Growth Factors and Angiogenesis in Pancreatic Islet Transplantation. Juvenile Diabetes Research Foundation International Suzanne Laychock $125,000

August 2004 to July 2006 Regulated Insulin Secretion from Tissue Engineered Skin for Type-1 Diabetes NIH Suzanne Laychock, Stelios Andreadis, Keith Kirkwood $75,000

August 2002 to July 2005 EDG Receptor and Sphingosine-1-Phosphate EFfects in Pancreatic Islets Juvenile Diabetes Research Foundation Suzanne Laychock $370,197

July 1998 to June 2001 Regulation of Expression of Receptors for Inositol Trisphosphate and Ryanodine in Pancreatic Islets American Diabetes Association Suzanne Laychock $150,000

September 1996 to April 1998 Diet-induced hyperinsulinemia and beta cell function National Institute of Diabetes and Digestive and Kidney Diseases Mulchand Patel, Suzanne Laychock $160,000

July 1979 to June 2002 Putative Mediators of Insulin Secretion NIH Suzanne Laychock $289,746